Service
Personal Care
Testing
Comprehensive in-vitro safety and efficacy studies for personal care products using advanced 2D and 3D models. We are committed to promoting cruelty-free practices in the cosmetics and chemical industry through cutting-edge technologies and alternative testing methods.
Our Commitment
Ethical Testing Solutions
for Personal Care
We are committed to promoting cruelty-free practices in the cosmetics and chemical industry. Our non-animal testing approach utilizes cutting-edge technologies and alternative testing methods that eliminate the need for animal experimentation, without compromising the accuracy and validity of the results.
By choosing our services, you can have complete confidence that your products have been tested ethically and responsibly.
Why Choose Us
Key Features
Discover what makes our personal care testing services stand out in the industry.
Cruelty-Free Testing
Promoting cruelty-free practices in the cosmetics and chemical industry with ethical testing methods.
Advanced Technologies
Utilizing cutting-edge technologies and alternative testing methods for superior results.
Accurate Results
Eliminating animal experimentation without compromising accuracy and validity of results.
Ethical Confidence
Complete confidence that your products have been tested ethically and responsibly.
Service Offerings
Comprehensive Solutions
From in-vitro studies to clinical trials, we provide end-to-end solutions for your personal care testing needs.
In-vitro Safety and Efficacy Studies
Comprehensive in-vitro safety and efficacy studies using advanced 2D and 3D models for personal care product testing.
Key Offerings:
2D models (Cell lines and Primary Cells)
3D models (Reconstructed Human Tissues)
Safety assessment protocols
Efficacy evaluation studies
Quality control testing
Regulatory compliance
Skin Care Studies
Advanced Platforms
We are committed to promoting cruelty-free practices in the cosmetics and chemical industry. Our non-animal testing approach utilizes cutting-edge technologies and alternative testing methods that eliminate the need for animal experimentation, without compromising the accuracy and validity of the results.
Advanced Platforms
Cutting-edge cell lines and tissue models for comprehensive testing
HaCaT
Human keratinocyte cell line for skin studies
Human Epidermal Keratinocytes (NHEK)
Primary human epidermal keratinocytes
Human Dermal Fibroblasts (NHDF)
Primary human dermal fibroblasts
Melanocyte cell lines (B16)
Mouse melanoma cell line for pigmentation studies
Keratinocytes and melanocytes (Co-Culture)
Co-culture system for complex skin studies
Human & Mouse Adipocytes
Adipocyte models for skin and body care studies
Induced Pluripotent Stem Cell
iPSC-derived cells for advanced studies
Comprehensive Testing
Skin Irritation & Allergy Studies
Comprehensive skin irritation and allergy studies using advanced in-vitro models to assess the safety and compatibility of personal care products. Our specialized testing protocols ensure thorough evaluation of potential skin reactions.
Skin Irritation - Non irritant
Products can be tested:
Moisturizers
Lotions and creams
Soaps
Oils
Anti-rash cream
Sunscreen
Shampoo
Wipes
Powder and fragrances
Viability of skin cells
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Reconstructed human Epidermis developed using Neonatal and adult cells | SI.VI.RHE.SP.001 | Irritation potential of test compounds against the human tissue models | Spectrophotometry |
| Reconstructed full thickness skin developed using Neonatal and adult cells | SI.VI.RFT.SP.001 | Irritation potential of test compounds against the human tissue models | Spectrophotometry |
Inflammation Studies/Allergy - Non-Allergic
Products can be tested:
Moisturizers
Lotions and creams
Soaps
Oils
Anti-rash cream
Sunscreen
Shampoo
Wipes
Powder and fragrances
Gene expression
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal keratinocytes - Neonatal and adult | IS.GE.NKC.QP.001 | mRNA gene expression or protein levels (TNF,IL1A,IL1B,IL6,IL8,IL10,IL17,ICAM,COX2,ALOX5,NFkB,AP1,STAT1,HO-1...) after external induction | qPCR |
| Reconstructed human Epidermis developed using Neonatal and adult cells | IS.GE.RHE.QP.001 | mRNA gene expression or protein levels (TNF,IL1A,IL1B,IL6,IL8,IL10,IL17,ICAM,COX2,ALOX5,NFkB,AP1,STAT1,HO-1...) after external induction | qPCR |
| Reconstructed full thickness skin developed using Neonatal and adult cells | IS.GE.RFT.QP.001 | mRNA gene expression or protein levels (TNF,IL1A,IL1B,IL6,IL8,IL10,IL17,ICAM,COX2,ALOX5,NFkB,AP1,STAT1,HO-1...) after external induction | qPCR |
Protein Synthesis
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal keratinocytes - Neonatal and adult | IS.PS.NKC.ELA.001 | mRNA gene expression or protein levels (TNF,IL1A,IL1B,IL6,IL8,IL10,IL17,ICAM,COX2,ALOX5,NFkB,AP1,STAT1,HO-1...) after external induction | ELISA |
| Reconstructed human Epidermis developed using Neonatal and adult cells | IS.PS.RHE.ELA.001 | mRNA gene expression or protein levels (TNF,IL1A,IL1B,IL6,IL8,IL10,IL17,ICAM,COX2,ALOX5,NFkB,AP1,STAT1,HO-1...) after external induction | ELISA |
| Reconstructed full thickness skin developed using Neonatal and adult cells | IS.PS.RFT.ELA.001 | mRNA gene expression or protein levels (TNF,IL1A,IL1B,IL6,IL8,IL10,IL17,ICAM,COX2,ALOX5,NFkB,AP1,STAT1,HO-1...) after external induction | ELISA |
Advanced Testing
Skincare Testing
Comprehensive skincare testing services covering cytotoxicity, permeation studies, microbiome analysis, sensitization, and phototoxicity assessment using advanced in-vitro models.
Skincare Testing
Advanced skin models for comprehensive safety and efficacy testing
CYTOTOXICITY AND PERMEATION STUDIES
Cytotoxicity - Non-cytotoxic
Products can be tested:
Moisturizers
Lotions and creams
Soaps
Oils
Anti-rash cream
Sunscreen
Shampoo
Wipes
Powder and fragrances
Test Method: MTT
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal keratinocytes - Neonatal and adult | CT.VI.NKC.AB.001 | Viability studies after treatment with test compounds | Spectrophotometry |
| Reconstructed human Epidermis developed using Neonatal and adult cells | CT.VI.RHE.AB.001 | Viability studies after treatment with test compounds | Spectrophotometry |
| Reconstructed full thickness skin developed using Neonatal and adult cells | CT.VI.RFT.AB.001 | Viability studies after treatment with test compounds | Spectrophotometry |
Skin Permeation
Products can be tested:
Moisturizers
Oils
Sunscreen
Lotions and creams
Test Method: Franz Diffusion
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Reconstructed human Epidermis developed using Neonatal and adult cells | SP.FD.RHE.PC.001 | Permeability coefficient | Franz Cells diffusion |
| Reconstructed full thickness skin developed using Neonatal and adult cells | SP.FD.RFT.PC.001 | Permeability coefficient | Franz Cells diffusion |
MICROBIOME, SENSITIZATION AND PHOTOTOXICITY
Skin Microbiome studies - Microbiome friendly
Products can be tested:
Moisturizers
Oils
Sunscreen
Lotions and creams
Powder
Test Method: Gene expression
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Reconstructed human Epidermis developed using Neonatal and adult cells | MS.GE.RHE.QP.001 | Good microbes protection to claim microbiome friendly qPCR to identify the pool | qPCR |
| Reconstructed full thickness skin developed using Neonatal and adult cells | MS.GE.RFT.QP.001 | Good microbes protection to claim microbiome friendly qPCR to identify the pool | qPCR |
Phototoxicity studies - Photoprotective
Products can be tested:
Sunscreen
Test Method: Spectrofluorimetry
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| 3T3 Cells | PS.SF.3T3.AB.001 | Phototoxic potential of a test substance induced by the excited chemical after exposure to light. The test evaluates photo-cytotoxicity by the relative reduction in viability of cells exposed to the chemical in the presence versus absence of light. | Absorbance |
| Human epidermal keratinocytes - Neonatal and adult | PS.SF.NKC.AB.001 | Phototoxic potential of a test substance induced by the excited chemical after exposure to light. The test evaluates photo-cytotoxicity by the relative reduction in viability of cells exposed to the chemical in the presence versus absence of light. | Absorbance |
| Human epidermal Fibroblasts - Neonatal and adult | PS.SF.NFB.AB.001 | Phototoxic potential of a test substance induced by the excited chemical after exposure to light. The test evaluates photo-cytotoxicity by the relative reduction in viability of cells exposed to the chemical in the presence versus absence of light. | Absorbance |
Skin Sensitization
Products can be tested:
Oils
Moisturizers
Lotions and creams
Powders & Wipes
Test Method: Spectrophotometry
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal Keratinocytes - Neonatal and adult | SS.SP.NKC.AB.001 | Sensitization potential of test compound | Absorbance |
SKIN PIGMENTATION
Skin Pigmentation - Skin lightening
Products can be tested:
Oils
Moisturizers
Lotions and creams
Powders & Wipes
Test Method: Spectrophotometry
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal Melanocytes - Neonatal and adult | SL.SP.NMC.AB.001 | Melanin concentration | Absorbance |
| Human epidermal Keratinocytes - Neonatal and adult | SL.SP.NKC.AB.001 | Melanin concentration | Absorbance |
Skin Pigmentation - Skin lightening
Products can be tested:
Oils
Moisturizers
Lotions and creams
Powders & Wipes
Test Method: Gene expression
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal Melanocytes - Neonatal and adult | SL.GE.NMC.QP.001 | Melanogenesis pathways (TYR, TYRP-1, TYRP-2, MITF, and POMC) | qPCR |
| Human epidermal Keratinocytes - Neonatal and adult | SL.GE.NKC.QP.001 | Melanogenesis pathways (TYR, TYRP-1, TYRP-2, MITF, and POMC) | qPCR |
SKIN HYDRATION TEST
Moisturizing and Hydration
Products can be tested:
Moisturizers
Lotions and creams
Soaps
Oils
Test Method: Protein Synthesis
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal keratinocytes | SH.PS.NKC.ELA.001 | Protein (filaggrin, involucrin, cytokeratins, integrin, occludin, claudin, connexins, collagens, desmoglein, B-catenin) or Biomolecules levels (hyaluronic acid, ceramides, GAGs...). | ELISA |
| Reconstructed human Epidermis developed using cells | SH.PS.RHE.ELA.001 | Protein (filaggrin, involucrin, cytokeratins, integrin, occludin, claudin, connexins, collagens, desmoglein, B-catenin) or Biomolecules levels (hyaluronic acid, ceramides, GAGs...). | ELISA |
| Reconstructed full thickness skin developed using cells | SH.PS.RFT.ELA.001 | Protein (filaggrin, involucrin, cytokeratins, integrin, occludin, claudin, connexins, collagens, desmoglein, B-catenin) or Biomolecules levels (hyaluronic acid, ceramides, GAGs...). | ELISA |
Moisturizing and Hydration
Products can be tested:
Moisturizers
Lotions and creams
Soaps
Oils
Test Method: Gene expression
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal keratinocytes - adult and Neonatal and adult and adult | SH.GE.NKC.QP.001 | mRNA expression of genes involved in moisturizing and epidermal barrier pathways (HAS1/2/3, HYAL1/2/3, AQP1/2/3). | qPCR |
| Reconstructed human Epidermis developed using adult and Neonatal and adult and adult cells | SH.GE.RHE.QP.001 | mRNA expression of genes involved in moisturizing and epidermal barrier pathways (HAS1/2/3, HYAL1/2/3, AQP1/2/3). | qPCR |
| Reconstructed full thickness skin developed using adult and Neonatal and adult and adult cells | SH.GE.RFT.QP.001 | mRNA expression of genes involved in moisturizing and epidermal barrier pathways (HAS1/2/3, HYAL1/2/3, AQP1/2/3). | qPCR |
Moisturizing and Hydration
Products can be tested:
Moisturizers
Lotions and creams
Soaps
Oils
Test Method: Immunofluorescence Analysis
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human epidermal keratinocytes - adult and Neonatal and adult and adult | SH.IF.NKC.IA.001 | Immunofluorescence analysis of biomolecules or proteins using specific antibodies, including DAPI staining for cell nuclei. | Immunofluorescence |
| Reconstructed human Epidermis developed using adult and Neonatal and adult and adult cells | SH.IF.RHE.IA.001 | Immunofluorescence analysis of biomolecules or proteins using specific antibodies, including DAPI staining for cell nuclei. | Immunofluorescence |
| Reconstructed full thickness skin developed using adult and Neonatal and adult and adult cells | SH.IF.RFT.IA.001 | Immunofluorescence analysis of biomolecules or proteins using specific antibodies, including DAPI staining for cell nuclei. | Immunofluorescence |
OTHER KEY STUDIES
Anti-oxidant Potential
Test Method: mRNA gene expression (NRF2, CAT, GPx1, SOD1)
Reference Code: AO.GE.ALL.QP.001
Technique: qPCR
Platforms:
NHK
NHF
RHE
RFT
Anti-oxidant Potential
Test Method: Reactive Oxygen Species, Reactive Nitrogen Species
Reference Code: AO.KT.ALL.AB.001
Technique: KIT based
Platforms:
NHK
NHF
RHE
RFT
Anti-oxidant Potential
Test Method: Glutathione levels
Reference Code: AO.GT.ALL.SP.001
Technique: Spectrophotometric method
Platforms:
NHK
NHF
RHE
RFT
Anti-pollution studies
Test Method: Cell viability after dust exposure
Reference Code: AP.CV.ALL.SP.001
Technique: MTT
Platforms:
NHK
NHF
RHE
RFT
Anti-pollution studies
Test Method: Oxidative Stress (ROS)
Reference Code: AP.OS.ALL.SP.001
Technique: KIT based
Platforms:
NHK
NHF
RHE
RFT
Anti-pollution studies
Test Method: Inflammatory mediators (TNFa, IL1a, IL6 and IL8)
Reference Code: AP.IL.ALL.ELA.001
Technique: ELISA
Platforms:
NHK
NHF
RHE
RFT
Anti-pollution studies
Test Method: Apoptosis-induced biomarkers (CASP9, BAX2, ARNTL...)
Reference Code: AP.AP.ALL.ELA/IF/QP.001
Technique: qPCR/ELISA/Immunofluorescence
Platforms:
NHK
NHF
RHE
RFT
Anti-pollution studies
Test Method: Xenobiotic markers (AhR-CYP1A1, NRF2)
Reference Code: AP.XM.ALL.ELA/IF/QP.001
Technique: qPCR/ELISA/Immunofluorescence
Platforms:
NHK
NHF
RHE
RFT
Anti-Ageing Studies
Test Method: Extracellular matrix proteins (Elastin, hyaluronic acid, collagen, versican, and MMPs)
Reference Code: AAS.ECM.ALL.IS.001
Technique: Immunostaining
Platforms:
NHK
NHF
RHE
RFT
Anti-Ageing Studies
Test Method: DNA damage, Oxidative stress
Reference Code: AAS.DNA.ALL.SP.001
Technique: ROS, MTT, thymine dimers, Y-H2AX assay
Platforms:
NHK
NHF
RHE
RFT
Anti-Ageing Studies
Test Method: Senescence markers (TEP1, telomeres, SIRT1, TERT, and B-gal)
Reference Code: AAS.SM.ALL.ELA/IF/QP.001
Technique: qPCR/ELISA/Immunofluorescence
Platforms:
NHK
NHF
RHE
RFT
Anti-Ageing Studies
Test Method: Cell cycle and autophagy markers (PER, CDKN1A, CLOCK, LC3, BMAL, LMNB1)
Reference Code: AAS.AM.ALL.IF/QP.001
Technique: qPCR/Immunofluorescence
Platforms:
NHK
NHF
RHE
RFT
Anti-Ageing Studies
Test Method: Proteasome activity (APP, clusterin, chymotrypsin-like)
Reference Code: AAS.PA.ALL.KT.001
Technique: KIT based
Platforms:
NHK
NHF
RHE
RFT
Anti Acne studies
Test Method: 1. Total Fatty Acid Content, 2. Lipogenesis mRNA expression, 3. Sebocyte differentiation
Reference Code: AC.OR.ALL.QP/IS.001
Technique: Immunostaining /qPCR
Platforms:
Acne induced Reconstructed Human Skin Epidermis (Cutibacterium Acnes)
Acne induced Full thickness Reconstructed Human Skin (Cutibacterium Acnes)
iPSC-derived sebocytes (induced Pluripotent Stem Cell)
Human Adipocytes (HPAd, HAd)
Mouse Adipocytes (3T3-L1)
Normal Human Dermal Fibroblasts (NHDF)
Haircare Testing
Haircare Studies
Comprehensive haircare testing services covering hair growth studies and anti-dandruff assessment using advanced in-vitro models and specialized hair follicle cells.
Haircare Testing
Advanced hair follicle and dermal papillary cell studies
HAIRGROWTH STUDIES
Hairgrowth studies
Products can be tested:
All Haircare products
Test Method: MTT - Proliferation
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human dermal papillary cells - adult | HS.VI.HDP.VI.001 | Cell viability | Absorbance |
Hairgrowth studies
Products can be tested:
All Haircare products
Test Method: Gene expression
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human dermal papillary cells - adult | HS.GE.HDP.QP.001 | mRNA gene expression growth factors (EDNI, IGFI, HDC, TGFb, VEGF, KGF, FGF1, FGF2, FGF10, EGF...) | qPCR |
Hairgrowth studies
Products can be tested:
All Haircare products
Test Method: Protein Synthesis
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human dermal papillary cells - adult | HS.PS.HDP.ELA.001 | 1. Proteins involved in pathway of proliferation (VEGF, EGF, IGFI, EDN1, FGF1, FGF2, FGF10...) 2. Keratin levels - Hair strength (KRT 31-40 and KRT 81-86) | Immunofluoroscences |
Hairgrowth studies
Products can be tested:
All Haircare products
Test Method: 5-alpha Reductase activity
| Model | Reference Code | Biomarker Endpoints | Technique |
|---|---|---|---|
| Human dermal papillary cells - adult | HS.PS.HDP.5AR.001 | 5-alpha Reductase (SRD5A2, SRD5S2, SRD5S3) activity | ELISA/Immunofluorescence |
ANTI-DANDRUFF STUDIES
Anti-Dandruff
Test Method: Minimum inhibitory concentration
Reference Code: HC.AD.DRHE.MIC
Technique: Microdilution assay
Platforms:
Dandruff induced RHE
Human Primary Keratinocytes infected with Malassezia Furfur
Anti-Dandruff
Test Method: Contact time of drug and microbes
Reference Code: HC.AD.DRHE.CDT
Technique: Contact duration testing
Platforms:
Dandruff induced RHE
Human Primary Keratinocytes infected with Malassezia Furfur
Anti-Dandruff
Test Method: Gene Expression analysis (IL-1RA, S100A8, S100A9, S100A11, IL-8)
Reference Code: HC.AD.DRHE.GE.RT
Technique: RT-PCR
Platforms:
Dandruff induced RHE
Human Primary Keratinocytes infected with Malassezia Furfur
Anti-Dandruff
Test Method: Secretome analysis (IL-1α, IL-6, IL-8 and TNF-α)
Reference Code: HC.AD.DRHE.SA.ELA
Technique: ELISA
Platforms:
Dandruff induced RHE
Human Primary Keratinocytes infected with Malassezia Furfur
Anti-Dandruff
Test Method: Barrier Integrity
Reference Code: HC.AD.DRHE.BI.TEER
Technique: TEER Value/Lucifer yellow
Platforms:
Dandruff induced RHE
Human Primary Keratinocytes infected with Malassezia Furfur
Femcare Testing
Femcare Testing
Comprehensive femcare testing services using advanced reconstructed vaginal epithelium models for safety, efficacy, and compatibility assessment of feminine care products.
Product
BIODVAG- RECONSTRUCTED VAGINAL EPITHELIUM
Replica of human vaginal epithelium
Study the effects of various agents
Safety and efficacy studies
Femcare Testing
Advanced vaginal epithelium models for safety assessment
Platforms
PRIMARY VAGINAL EPITHELIAL CELLS
RECONSTRUCTED HUMAN VAGINAL EPITHELIUM
A431 CELL LINE
Services
| Study Name | Platforms | Claims |
|---|---|---|
| Vaginal Irritation | Reconstructed Human vaginal epithelium | Irritation Free Products |
| Cytotoxicity | A431 Cell lines, Primary vaginal epithelial cells | Irritation Free Products |
| Allergy/inflammation studies | Reconstructed Human vaginal epithelium, A431 Cell lines, Primary vaginal epithelial cells | Non-allergic |
| Microbiome/pathogen | Reconstructed Human vaginal epithelium | Microbiome Friendly |
| Hydration (Moisturizers) | Reconstructed Human vaginal epithelium | Keeps hydrated |
| Genetic toxicity | Primary vaginal epithelium and A431 Cell line | Non Mutagenic |
Products Can Be Tested
Menstrual Pads
Tampons
Menstrual Cups
Panty Liners
Intimate Wipes
Feminine Wash
Feminine Powder
Feminine Deodorant
Feminine Moisturizers
Vaginal Itch Creams
Menstrual Pain Relief Products
Lubricants
Computational Testing
IN-SILICO TESTING
We offer wide range of in-silico services to our customers for their screening and product development needs using advanced computational methods.
Product Development
- in-silico virtual screening
- Identifying active molecules
- Product repurposing
- Selecting the most relevant in vitro assays
Efficacy Studies
- Skin penetration studies
- Molecular Dynamics
- Mechanistic prediction of solute properties in bio-membranes
Computational Analysis
Advanced molecular modeling and virtual screening
Key Studies
Molecular Docking
Virtual Screening
QSAR (Quantitative Structure-Activity Relationship)
ADME Studies
Permeation studies (Skin)
Toxicity Prediction
Formulation Optimization
Protein-Ligand Interaction Studies
Structural Characterization
Particle-Particle Interactions
Molecular Dynamics Simulations
Predicting Photostability
Predicting Fragrance Interactions
Solubility and Partition Coefficient Calculations
Software & Tools
3DS Biovia - Material Studio, Discovery Studio, Cosmotherm & plex
Clinical Testing
CLINICAL TESTING
- HRIPT (Dermatologically tested, Clinically tested, Non-irritant, Hypoallergenic and tested under a dermatologist's supervision), 37 days
- Patch Test (Non-irritant and tested under a dermatologist's supervision) 48hrs/72 hrs test
Alternative to Human Clinical Studies
Testing on Reconstructed Human Tissues, which is the functional replica of native human tissues and provides human relevant data similar to the clinical testing.
COMPARISON CHART
| in vitro | 3D Human Tissue | Clinical - Human | |
|---|---|---|---|
| Endpoints (PATCH) | Irritation and Inflammation (quantification) | Irritation, allergy, inflammation and biomarker quantification | Visual inspection for irritation and inflammation |
| Cost | 1-1.5 lakhs | 1.5 - 2.5 lakhs | 25,000 - 2 lakhs |
| Advantage |
|
|
|
| Disadvantage | Basic understanding of product's safety and toxicity | High cost |
|
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Personal Care Testing
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